
Accessories and replacement parts for EC columns
Analytical column system manufactured from stainless steel M 8 outer threads on both ends combination of sealing element and very fine-meshed stainless steel screen, PTFE sealing ring and fitting adaptor column heads SW 12 with inner threads M8 x 0,75 and UNF 10-32, as built -in guard columns use ChromCart® guard column cartridges with 8mm length with the guard column adaptor EC, packed with NUCLEODUR® spherical silica.
EC analytical columns NUCLEOCEL DELTA S, 5 µm
NUCLEOCEL DELTA enantiomer separation based on cellulose derivativesBase material silica,chiral selector Cellulosetris-(3,5-dimethylphenylcarbamate) - USP L40Similar phases: Chiralcel® OD, Kromasil®, CelluCoat™, Eurocel® 01S-Type for high resolution, allows use of shorter columns (150 mm) for faster separations, pressure stability up to ~ 150 bar (2000 psi).Recommended applications: pharmaceutically active compounds, chiral pollutants (e. g. herbicides, PCB), chiral compounds in food (dyes, preservatives), chiral catalysts and bioorganic compounds.NUCLEOCEL DELTA for normal phase applications:Eluent in column n-heptane - propanol-2 (90:10, v/v), particle size 5 µm.
EC analytical columns NUCLEOCEL DELTA-RP S, 5 µm
NUCLEOCEL DELTA enantiomer separation based on cellulose derivativesBase material silica,chiral selector Cellulosetris-(3,5-dimethylphenylcarbamate) - USP L40Similar phases: Chiralcel® OD, Kromasil®, CelluCoat™, Eurocel® 01S-Type for high resolution, allows use of shorter columns (150 mm) for faster separations, pressure stability up to ~ 150 bar (2000 psi).Recommended applications: pharmaceutically active compounds, chiral pollutants (e. g. herbicides, PCB), chiral compounds in food (dyes, preservatives), chiral catalysts and bioorganic compounds.NUCLEOCEL DELTA-RP for reversed phase applications:Eluent in column acetonitrile - water (40:60, v/v), particle size 5 µm. Designed for use either in polar organic mode or with eluents containing high concentrations of chaotropic salts such as perchlorate.

EC analytical columns NUCLEODUR® 100-3
Totally spherical high purity silica - USP L3 pore size 110 Å, pore volume 0.9 ml/g, surface (BET) 340 m²/g, density 0.47 g/ml, pressure stability 800 bar, pH stability 2 - 8.Particle size 3 µm.

EC analytical columns NUCLEODUR® 100-3 C18 ec, 3 µm
Available with medium density octadecyl (C18 - USP L1) and octyl (C8 - USP L7) modification pore size 110 Å, particle sizes 3 µm and 5 µm; 7 µm, 10 µm, 12 µm, 16 µm, 20 µm, 30 µm und 50 µm for preparative separations on request for daily routine analysis and up-scaling for preparative HPLC, pH stability 1 - 9. Carbon content 17.5 % C for C18, 10.5 % C for C8, high reproducibility from lot to lot for standard routine applications in reversed phase chromatography.Octadecyl phases, 17.5 % C, particle size 3 µm.

EC analytical columns NUCLEODUR® 100-3 C8 ec, 3 µm
Available with medium density octadecyl (C18 - USP L1) and octyl (C8 - USP L7) modification pore size 110 Å, particle sizes 3 µm and 5 µm; 7 µm, 10 µm, 12 µm, 16 µm, 20 µm, 30 µm und 50 µm for preparative separations on request for daily routine analysis and up-scaling for preparative HPLC, pH stability 1 - 9. Carbon content 17.5 % C for C18, 10.5 % C for C8, high reproducibility from lot to lot for standard routine applications in reversed phase chromatography.Octyl phases, 10.5 % C, particle size 3 µm.

EC analytical columns NUCLEODUR® 100-3 CN-RP, 3 µm
Pore size 110 Å, - USP L10Multi-mode columns (RP and NP)Widens the scope in selectivityDiffernet retention characteristics compared to C8 and C18Stable against hydrolysis at low pH values, working range pH 1-8High reproducibility from lot to lotIdeal for: tricyclic antidepressants, steroids, organic acidsEluent in column acetonitrile, particle size 3 µm.

EC analytical columns NUCLEODUR® 100-3 NH2-RP, 3 µm
Pore size 110 Å, particle sizes 3 and 5 µm; 2.5 % C; not endcapped - USP L8Multi-mode columns (RP and NP):Normal phase chromatography (NP) with hexan, dichloromethane or 2-propanol as mobile phase for polar compounds such as substituted anilines, esters, chlorinated pesticidesReversed phase chromatography (RP) of polar compounds like sugars in aqueous-organic eluent systemsIon exchange chromatography of anions and organic acids using common buffers and organic modifiersstable against hydrolysis at low pH, working range pH 2-8, 100 % stable in water, suitable for LC-MSIdeal for:polar compounds under RP conditions (sugars, DNA bases), hydrocarbons under NP conditionsEluent in column is n-heptane for the NP mode - RP columns are delivered in acetonitrile - water. For changing the solvent system a rinsing step with THF may be necessary.Eluent in column acetonitrile, particle size 3 µm.

EC analytical columns NUCLEODUR® 100-5
Totally spherical high purity silica - USP L3 pore size 110 Å, pore volume 0.9 ml/g, surface (BET) 340 m²/g, density 0.47 g/ml, pressure stability 800 bar, pH stability 2 - 8.Particle size 5 µm.

EC analytical columns NUCLEODUR® 100-5 C18 ec, 5 µm
Available with medium density octadecyl (C18 - USP L1) and octyl (C8 - USP L7) modification pore size 110 Å, particle sizes 3 µm and 5 µm; 7 µm, 10 µm, 12 µm, 16 µm, 20 µm, 30 µm und 50 µm for preparative separations on request for daily routine analysis and up-scaling for preparative HPLC, pH stability 1 - 9. Carbon content 17.5 % C for C18, 10.5 % C for C8, high reproducibility from lot to lot for standard routine applications in reversed phase chromatography.Octadecyl phases, 17.5 % C, particle size 5 µm.

EC analytical columns NUCLEODUR® 100-5 C8 ec, 5 µm
Available with medium density octadecyl (C18 - USP L1) and octyl (C8 - USP L7) modification pore size 110 Å, particle sizes 3 µm and 5 µm; 7 µm, 10 µm, 12 µm, 16 µm, 20 µm, 30 µm und 50 µm for preparative separations on request for daily routine analysis and up-scaling for preparative HPLC, pH stability 1 - 9. Carbon content 17.5 % C for C18, 10.5 % C for C8, high reproducibility from lot to lot for standard routine applications in reversed phase chromatography.Octyl phases, 10.5 % C, particle size 5 µm.

EC analytical columns NUCLEODUR® 100-5 CN, 5 µm
Pore size 110 Å, - USP L10Multi-mode columns (RP and NP)Widens the scope in selectivityDiffernet retention characteristics compared to C8 and C18Stable against hydrolysis at low pH values, working range pH 1-8High reproducibility from lot to lotIdeal for: tricyclic antidepressants, steroids, organic acidsEluent in column n-heptane, particle size 5 µm.

EC analytical columns NUCLEODUR® 100-5 CN-RP, 5 µm
Pore size 110 Å, - USP L10Multi-mode columns (RP and NP)Widens the scope in selectivityDiffernet retention characteristics compared to C8 and C18Stable against hydrolysis at low pH values, working range pH 1-8High reproducibility from lot to lotIdeal for: tricyclic antidepressants, steroids, organic acidsEluent in column acetonitrile, particle size 5 µm

EC analytical columns NUCLEODUR® 100-5 NH2, 5 µm
Pore size 110 Å, particle sizes 3 and 5 µm; 2.5 % C; not endcapped - USP L8Multi-mode columns (RP and NP):Normal phase chromatography (NP) with hexan, dichloromethane or 2-propanol as mobile phase for polar compounds such as substituted anilines, esters, chlorinated pesticidesReversed phase chromatography (RP) of polar compounds like sugars in aqueous-organic eluent systemsIon exchange chromatography of anions and organic acids using common buffers and organic modifiersstable against hydrolysis at low pH, working range pH 2-8, 100 % stable in water, suitable for LC-MSIdeal for:polar compounds under RP conditions (sugars, DNA bases), hydrocarbons under NP conditionsEluent in column is n-heptane for the NP mode - RP columns are delivered in acetonitrile - water. For changing the solvent system a rinsing step with THF may be necessary.Eluent in column n-heptane, particle size 5 µm.

EC analytical columns NUCLEODUR® 100-5 NH2-RP, 5 µm
Pore size 110 Å, particle sizes 3 and 5 µm; 2.5 % C; not endcapped - USP L8Multi-mode columns (RP and NP):Normal phase chromatography (NP) with hexan, dichloromethane or 2-propanol as mobile phase for polar compounds such as substituted anilines, esters, chlorinated pesticidesReversed phase chromatography (RP) of polar compounds like sugars in aqueous-organic eluent systemsIon exchange chromatography of anions and organic acids using common buffers and organic modifiersstable against hydrolysis at low pH, working range pH 2-8, 100 % stable in water, suitable for LC-MSIdeal for:polar compounds under RP conditions (sugars, DNA bases), hydrocarbons under NP conditionsEluent in column is n-heptane for the NP mode - RP columns are delivered in acetonitrile - water. For changing the solvent system a rinsing step with THF may be necessary.Eluent in column acetonitrile, particle size 5 µm.
EC analytical columns NUCLEODUR® C18 Gravity, 1.8 µm
NUCLEODUR® C18 - C 8 Gravity nonpolar high density phasesAvailable as octadecyl (C18 -USP L1) and octyl (C8 - USP L7) modifications. Pore size 110 Å.Ideal for method developmentAllows HPLC at pH extremes (pH 1 - 11)Suitable for LC/MS due to low bleeding characteristicsRecommended for overall sophisticated analytical separationsOptimal for: pharmaceuticals, e.g. analgesics, antiinflammatory drugs, antidepressants, herbicides, phytopharmaceuticals, immunosppressantsParticle size 1.8 µm, 18 % C
EC analytical columns NUCLEODUR® C18 Gravity, 3 µm
NUCLEODUR® C18 - C 8 Gravity nonpolar high density phasesAvailable as octadecyl (C18 -USP L1) and octyl (C8 - USP L7) modifications. Pore size 110 Å.Ideal for method developmentAllows HPLC at pH extremes (pH 1 - 11)Suitable for LC/MS due to low bleeding characteristicsRecommended for overall sophisticated analytical separationsOptimal for: pharmaceuticals, e.g. analgesics, antiinflammatory drugs, antidepressants, herbicides, phytopharmaceuticals, immunosppressantsParticle size 3 µm, 18 % C.

EC analytical columns NUCLEODUR® C18 Gravity, 5 µm
NUCLEODUR® C18 - C 8 Gravity nonpolar high density phasesAvailable as octadecyl (C18 -USP L1) and octyl (C8 - USP L7) modifications. Pore size 110 Å.Ideal for method developmentAllows HPLC at pH extremes (pH 1 - 11)Suitable for LC/MS due to low bleeding characteristicsRecommended for overall sophisticated analytical separationsOptimal for: pharmaceuticals, e.g. analgesics, antiinflammatory drugs, antidepressants, herbicides, phytopharmaceuticals, immunosppressantsParticle size 5 µm, 18 % C

EC analytical columns NUCLEODUR® C18 Isis, 1.8 µm
C18 phase with special polymeric, crosslinked surface modification - USP L1Pore size 110 ÅHigh steric selectivityOutstanding surface deactivationSuitable for LC/MS due low bleeding characteristicspH stability 1 - 10Broad range of application: steroids, (o,p,m-) substituted aromatics, fat-soluble vitaminsParticle size 1.8 µm, 20 % C

EC analytical columns NUCLEODUR® C18 Isis, 3 µm
C18 phase with special polymeric, crosslinked surface modification - USP L1Pore size 110 ÅHigh steric selectivityOutstanding surface deactivationSuitable for LC/MS due low bleeding characteristicspH stability 1 - 10Broad range of application: steroids, (o,p,m-) substituted aromatics, fat-soluble vitaminsParticle size 3 µm, 20 % C

EC analytical columns NUCLEODUR® C18 Isis, 5 µm
C18 phase with special polymeric, crosslinked surface modification - USP L1Pore size 110 ÅHigh steric selectivityOutstanding surface deactivationSuitable for LC/MS due low bleeding characteristicspH stability 1 - 10Broad range of application: steroids, (o,p,m-) substituted aromatics, fat-soluble vitaminsParticle size 5 µm, 20 % C

EC analytical columns NUCLEODUR® C18 Pyramid, 1.8 µm
Stable in 100 % aqueous eluent systems- USP L1Pore size 110 ÅInteresting polar selectivity featuresExcellent base deactivation; suitable for LC/MS due low bleeding characteristicspH stability 1 - 9Ideal for: analgesics, penicillin antibiotics, nucleic acid bases, water-soluble vitamins, complexing agetns, organic acidsParticle size 1.8 µm, 14 % C.

EC analytical columns NUCLEODUR® C18 Pyramid, 3 µm
Stable in 100 % aqueous eluent systems- USP L1Pore size 110 ÅInteresting polar selectivity featuresExcellent base deactivation; suitable for LC/MS due low bleeding characteristicspH stability 1 - 9Ideal for: analgesics, penicillin antibiotics, nucleic acid bases, water-soluble vitamins, complexing agetns, organic acidsParticle size 3 µm, 14 % C

EC analytical columns NUCLEODUR® C18 Pyramid, 5 µm
Stable in 100 % aqueous eluent systems- USP L1Pore size 110 ÅInteresting polar selectivity featuresExcellent base deactivation; suitable for LC/MS due low bleeding characteristicspH stability 1 - 9Ideal for: analgesics, penicillin antibiotics, nucleic acid bases, water-soluble vitamins, complexing agetns, organic acidsParticle size 5 µm, 14 % C
EC analytical columns NUCLEODUR® C8 Gravity, 1.8 µm
NUCLEODUR® C18 - C 8 Gravity nonpolar high density phasesAvailable as octadecyl (C18 -USP L1) and octyl (C8 - USP L7) modifications. Pore size 110 Å.Ideal for method developmentAllows HPLC at pH extremes (pH 1 - 11)Suitable for LC/MS due to low bleeding characteristicsRecommended for overall sophisticated analytical separationsOptimal for: pharmaceuticals, e.g. analgesics, antiinflammatory drugs, antidepressants, herbicides, phytopharmaceuticals, immunosppressantsParticle size 1.8 µm, 11 % C.

EC analytical columns NUCLEODUR® C8 Gravity, 5 µm
NUCLEODUR® C18 - C 8 Gravity nonpolar high density phasesAvailable as octadecyl (C18 -USP L1) and octyl (C8 - USP L7) modifications. Pore size 110 Å.Ideal for method developmentAllows HPLC at pH extremes (pH 1 - 11)Suitable for LC/MS due to low bleeding characteristicsRecommended for overall sophisticated analytical separationsOptimal for: pharmaceuticals, e.g. analgesics, antiinflammatory drugs, antidepressants, herbicides, phytopharmaceuticals, immunosppressantsParticle size 5 µm, 11 % C

EC analytical columns NUCLEODUR® Sphinx RP, 1.8 µm
Distinct selectivity based on bifunctional surface coverage - USP L1 and USP L11Pore size 110 ÅHigh density of covalently bonded silanes for tailing-free peaks widens the scope for method developmentpH stability 1 - 10Suitable for LC/MS due low bleeding characteristicsHigh reproducibility and consistent quality due to tight QC proceduresRange of application: quinolone antibiotics, sulfonamides, xanthines, substituted aromaticsParticle size 1.8 µm, 15 % C

EC analytical columns NUCLEODUR® Sphinx RP, 3 µm
Distinct selectivity based on bifunctional surface coverage - USP L1 and USP L11Pore size 110 ÅHigh density of covalently bonded silanes for tailing-free peaks widens the scope for method developmentpH stability 1 - 10Suitable for LC/MS due low bleeding characteristicsHigh reproducibility and consistent quality due to tight QC proceduresRange of application: quinolone antibiotics, sulfonamides, xanthines, substituted aromaticsParticle size 3 µm, 15 % C

EC analytical columns NUCLEODUR® Sphinx RP, 5 µm
Distinct selectivity based on bifunctional surface coverage - USP L1 and USP L11Pore size 110 ÅHigh density of covalently bonded silanes for tailing-free peaks widens the scope for method developmentpH stability 1 - 10Suitable for LC/MS due low bleeding characteristicsHigh reproducibility and consistent quality due to tight QC proceduresRange of application: quinolone antibiotics, sulfonamides, xanthines, substituted aromaticsParticle size 5 µm, 15 % C
EC analytical columns NUCLEOSIL® 100-5 C18 PAH, 5 µm
Special octadecyl phase for PAH analysis.Base material NUCLEOSIL® silica, particle size 5 µm, pore size 100 Å; polymeric coating - USP L1; eluent in column acetonitrile/water 70:30; allows efficient gradient separation of the 16 PAH in accordance with EPA.Detection of the separated PAH by UV (250 to 280 nm), with diode array or with fluorescence detection at different wavelegths for excitation and emission (acenaphthylene cannot be analysed with fluorescence detection).Guard columns for EC columns NUCLEOSIL® 100-5 C18 PAH, 5 µm available on request.

EC analytical HPLC columns, NUCLEODUR PFAS
Hydrophobic reversed phase with distinct polar selectivity for special PFAS analysis. This HPLC phase is made of high purity NUCLEODUR HPLC silica gel and is extensively endcapped. Due to a low bleeding characteristics these columns are suitable for high sensitivity LC/MS analysis.

EC analytical HPLC columns, NUCLEODUR PFAS Delay
NUCLEODUR PFAS Delay columns trap and therefore delay system-related PFAS contaminants, ensuring trace level analysis of PFAS in your samples. This HPLC phase is made of high purity NUCLEODUR HPLC silica gel and is extensively endcapped. Due to a low bleeding characteristics these columns are suitable for high sensitivity LC/MS analysis.

Guard columns for EC columns NUCLEODUR® C18 Gravity, 3µm
NUCLEODUR® C18 - C 8 Gravity nonpolar high density phasesavailable as octadecyl (C18 -USP L!) and octyl (C8 - USP L7) modificationsPore size 110 Å; particle sizes 1,8µm, 3µm and 5µm for C18, 1,8 and 5µm for C87, 10, 12 and 16µm particles for preparative separations on requestcarbon content 18 %C for C18 , 11%C for C8ideal for method developmentallows HPLC at pH extremes (pH 1 - 11)suitable for LC/MS due to low bleeding characteristicsrecommended for overall sophisticated analytical separationsoptimal for: pharmaceuticals, e.g. analgesics, antiinflammatory drugs, antidepressants; herbicides; phytopharmaceuticals; immunosppressantsGuard columns for EC columns require guard column adapter EC (Cat. No. 7.081 898)
Guard columns for EC columns NUCLEODUR® C18 Isis, 3 µm
C18 phase with special polymeric, crosslinked surface modification - USP L1Pore size 110 ÅHigh steric selectivityOutstanding surface deactivationSuitable for LC/MS due low bleeding characteristicspH stability 1 - 10Broad range of application: steroids, (o,p,m-) substituted aromatics, fat-soluble vitaminsGuard columns for EC columns require the guard column adapter EC.
Guard columns for EC columns NUCLEODUR® C18Gravity, 5µm
NUCLEODUR® C18 - C 8 Gravity nonpolar high density phasesavailable as octadecyl (C18 -USP L!) and octyl (C8 - USP L7) modificationsPore size 110 Å; particle sizes 1,8µm, 3µm and 5µm for C18, 1,8 and 5µm for C87, 10, 12 and 16µm particles for preparative separations on requestcarbon content 18 %C for C18 , 11%C for C8ideal for method developmentallows HPLC at pH extremes (pH 1 - 11)suitable for LC/MS due to low bleeding characteristicsrecommended for overall sophisticated analytical separationsoptimal for: pharmaceuticals, e.g. analgesics, antiinflammatory drugs, antidepressants; herbicides; phytopharmaceuticals; immunosppressantsGuard columns for EC columns require guard column adapter EC (Cat. No. 7.081 898)