Electrofusion and electroporation system ECM® 2001+, Hybridoma production system
Multifunctional system for fast, efficient cell fusion in hybridoma production, hybrid cell formation, and nuclear transfer, as well as square wave electroporation for transfection of genes and other molecules into mammalian cell lines.Large, easy-to-use touchscreen interfacePreset protocols for common hybridoma, embryo manipulation, and nuclear transfer applicationsSeparate electrofusion and electroporation function menus for optimised protocol programming, data organisation, and navigationQuick start menus for fast access to the most frequently used electrofusion and electroporation parametersUser-defined electrofusion protocols: combination of up to 19 AC protocol steps and 1 DC protocol stepStorage of up to 1000 user-defined protocolsThe coaxial chambers have identical electrical characteristics to allow direct scale-up to production once the pulse parameters have been optimised. Coaxial optimisation chamber with transparent bottom allows visualisation of the cell alignment by inverted or regular microscope.Scope of supply: Generator ECM® 2001+, coaxial optimisation chamber (2 ml), coaxial production chamber (9 ml), adapter set, cytofusion medium C (500 ml)Square wave (DC)Voltage LV mode:5 ... 500 VVoltage HV mode:505 ... 3000 VMultiple pulse:1 ... 100 pulses per samplePulse length LV mode:10 µs ... 999 msPulse length HV mode:10 µs ... 600 µsPulse interval:0.1 ... 10 sSine wave (AC)Voltage:5 ... 75 VFrequency:0.2 ... 2 MHzPulse length:0 ... 99 sCharging time:7 s
Electrophoresis package MSCHOICETRIO-PP300
Combination package for any horizontal electrophoresis application. Ideal for starting work on electrophoresis techniques.Electrophoresis tank multiSUB Choice offers a selection of 3 gel tray sizes (15 x 7 cm, 15 x 10 cm and 15 x 15 cm) and can thus be used flexiblyPower supply PowerPro can be operated in constant mode or programmed and has 5 output pairsScope of supply: Electrophoresis tank multiSUB Choice TRIO, power supply PowerPro 300
Electrophoresis package MSMAXIDUO-PP300
Combination package for any horizontal electrophoresis application. Ideal for starting work on electrophoresis techniques.Electrophoresis tank multiSUB Maxi allows very accurate separation of DNA samples due to its long run and is equipped with 2 gel tray sizes (20 x 10 cm and 20 x 20 cm)Power supply PowerPro can be operated in constant mode or programmed and has 5 output pairsScope of supply:MSMAXIDUO-PP300: Electrophoresis tank multiSUB Maxi DUO, power supply PowerPro 300MSMAXIDUO-PP300-Kit: Electrophoresis tank multiSUB Maxi DUO, power supply PowerPro 300, agarose (100 g), DNA ladder (250 bp to 10 Kb), DNA loading dye Bromophenol blue, TBE-running buffer powder (10 x 1 l)
Electrophoresis package MSMIDI96-PP300
Combination package for any horizontal electrophoresis application. Ideal for starting work on electrophoresis techniques.Electrophoresis tank multiSUB Midi-96 is specially designed for loading DNA samples from multiwell plates. Samples from 96 well plates can be loaded directly with 8-channel pipettes.Power supply PowerPro can be operated in constant mode or programmed and has 5 output pairsScope of supply:MSMIDI96-PP300: Electrophoresis tank multiSUB Midi-96, power supply PowerPro 300MSMIDI96-PP300-Kit: Electrophoresis tank multiSUB Midi-96, power supply PowerPro 300, agarose (100 g), DNA ladder (250 bp to 10 Kb), DNA loading dye Bromophenol blue, TBE-running buffer powder (10 x 1 l)
Electrophoresis package MSMIDIDUO-NANO300
Combination package for any horizontal electrophoresis application. Ideal for starting work on electrophoresis techniques.Electrophoresis tank multiSUB Midi can process up to 100 samples and each platinum electrode is recessed in a removable cassette to protect it from damagePower supply nanoPAC with two-step function for changing the programming of voltage, current and time during the runScope of supply: Electrophoresis tank multiSUB Midi DUO, power supply nanoPAC-300P
Electrophoresis package MSMINIDUO-NANO300
Combination package for any horizontal electrophoresis application. Ideal for starting work on electrophoresis techniques.Electrophoresis tank multiSUB Mini can process up to 64 samples and is equipped with 2 gel tray sizes (7 x 7 cm and 7 x 10 cm)Power supply nanoPAC with two-step function for changing the programming of voltage, current and time during the runScope of supply:MSMINIDUO-NANO300: Electrophoresis tank multiSUB Mini DUO, power supply nanoPAC-300PMSMINIDUO-NANO300-Kit: Electrophoresis tank multiSUB Mini DUO, power supply nanoPAC-300P, agarose (100 g), DNA ladder (250 bp to 10 Kb), DNA loading dye Bromophenol blue, TBE-running buffer powder (10 x 1 l)
Electrophoresis package MSSCREENTRIO-PP500
Combination package for any horizontal electrophoresis application. Ideal for starting work on electrophoresis techniques.Electrophoresis tank multiSUB Screen offers a selection of 3 gel tray sizes (26 x 16 cm, 26 x 24 cm and 26 x 32 cm) and is equipped with buffer recirculationPower supply PowerPro can operate agarose gels at up to 500 V and has 5 output pairsScope of supply: Electrophoresis tank multiSUB Screen TRIO, power supply PowerPro 500
Electrophoresis System Enduro™ Gel XL
A complete electrophoresis system for fast and easy analysis. For 6 x 6 cm to 12.5 x 12 cm gels.Up to 112 samples at onceCasting set and combs includedSet voltage, amperage and timeTimer with end of run audible alertDirect connection eliminates leadsBacklit LCDSet includes: Enduro™ Gel XL, 1 main casting stand, 4 Micro, 6 x 6 cm gel trays, 1 tray divider, 2 double sided combs (5/8 well)Specifications:Output voltage/current10 to 150V / 10 to 400mATimer0 to 99 hours or continuousDimensions (W x D x H)245 x 170 x 62 mmGel Capacity1 large (12.5 x 12 cm), 2 small (12.5 x 6 cm), 4 micro (6 x 6 cm)Program memoryLast programmed parameters saved
Electroporation cuvettes Plus
BTX Cuvettes Plus are designed for use in electroporation and electrofusion of bacteria, yeast, insect, plant and mammalian cells. Each sterilized Cuvettes Plus package includes a disposable cuvette and a transfer pipette, which is used for removal of the sample after electroporation. The cuvettes are molded with embedded polished aluminum electrodes, and gamma irradiated for guaranteed sterility.Three electrode gap sizes are available:1mm for bacteria and yeast2mm for all cell types4mm for mammalian cellsBTX cuvette Plus can obtain high field strengths up to 25.0 kV/cm. Round caps are leak resistant and allow for quick and easy removal. For research use only. Can be used with electroporation systems from different manufacturers (not suitable for units from Eppendorf).
Electroporation System ECM® 399
The ECM® 399 is an electroporation system for the simple transformation of bacteria and yeast cells. It produces exponential decay waves and is designed to deliver the field strengths and pulse lengths required for transformations. The ECM® 399 is ideal for basic transformation in research and academic environments. It is easy to operate, cost effective, compact in size and portable.Applications: Transformation of bacteria, yeast, fungus.Scope of supply: Generator ECM® 399 , PEP cuvette holder, Cuvettes 1 mm, 2 mm, 4 mm (10 each) and Cuvette RackSpecificationsCapacity/Resistance Range:LV Mode: HV Mode:1050 µF / 150 Ohm, fixed 36 µF / 150 Ohm, fixedMax. Voltage Output:2500 V (Peak)Pulse Interval: Charge Time:100 ms to 10 s max. 5 sMax. Pulse Length:125 ms at 500 V (Peak) or 5 ms at 2500 V (Peak)Display:16-character, liquid crystal, LCD backlitInterface: Relative Humidity:Digital User Interface 60 %Power supply:100 - 240 V, 50 / 60 Hz
Electroporation system ECM® 830
The versatile electroporation system is suitable for bacterial and yeast cell transformations, as well as for transfections of mammalian cells and tissues. The supplied safety dome connects to the generator to safely deliver high-voltage electrical pulses to cuvettes. Up to two cuvettes can be electroporated simultaneously.Large, easy-to-use touchscreen interfacePreset protocols for commonly used mammalian cell lines and tissuesStorage of up to 1000 user-defined protocolsSafety functions: Pre-pulse resistance check, arc protection, and over-current pulse abortProtocol of each delivered pulse for quality control and troubleshootingScope of supply: Generator ECM® 830, safety dome, electroporation cuvettes 1 mm / 2 mm / 4 mm (10 each), cuvette rackCapacitance LV mode:3775 µFCapacitance HV mode:85 µFVoltage LV mode:5 ... 500 VVoltage HV mode:505 ... 3000 VMultiple pulse:1 ... 99 pulses per samplePulse length LV mode:10 µs ... 999 msPulse length HV mode:10 µs ... 600 µsPulse interval:100 ms ... 10 sCharging time LV mode:7 sCharging time HV mode:4 s
Electroporation system Gemini Twin Wave SC, Complete system
The Gemini Twin Wave devices are flexible systems that can generate both square wave and exponential decay waves. Ideally suited for all electroporation requirements. The waveform combination allows eukaryotic and prokaryotic cells to be electroporated easily and efficiently in cuvettes with a single device. Prokaryotic cells typically respond well to exponential decay wave pulses, while eukaryotic cells are most efficiently transfected with square wave pulses.Large, user-friendly touchscreen interfaceUniversal electroporation for transfection of cells in vitro, in vivo, in ovo, and adherent formsPreset protocols include the most common eukaryotic and prokaryotic cell typesUser-defined protocols: protocols can be added and modified without limitsSafety functions: Pre-pulse resistance check, arc protection, and over-current pulse abortData protocols store parameters of all delivered pulses for quality control and troubleshooting purposesStorage of over 1000 protocolsThe supplied safety dome connects to the generator to safely deliver high-voltage electrical pulses to cuvettes. Up to two cuvettes can be electroporated simultaneously.Scope of supply: Generator Gemini SC, safety dome, electroporation cuvettes 1 mm / 2 mm / 4 mm (10 each), cuvette rackExponential decay pulseCapacitance LV mode:25 ... 3275 µFCapacitance HV mode:10, 25, 50 µFResistance:50 ... 1000 ΩVoltage LV mode:10 ... 500 VVoltage HV mode:510 ... 3000 VSquare wave pulseCapacitance LV mode:3775 µFCapacitance HV mode:85 µFVoltage LV mode:10 ... 500 VVoltage HV mode:510 ... 3000 VMultiple pulse LV mode:1 ... 100 pulses per sampleMultiple pulse HV mode:1 ... 2 pulses per samplePulse length LV mode:0.05 ... 100 msPulse length HV mode:50 µs ... 5 msPulse interval:0.1 ... 10 sCharging time LV mode:7 sCharging time HV mode:4 s
Electroporation system Gemini Twin Wave SC, Generator
The Gemini Twin Wave devices are flexible systems that can generate both square wave and exponential decay waves. Ideally suited for all electroporation requirements. The waveform combination allows eukaryotic and prokaryotic cells to be electroporated easily and efficiently in cuvettes with a single device. Prokaryotic cells typically respond well to exponential decay wave pulses, while eukaryotic cells are most efficiently transfected with square wave pulses.Large, user-friendly touchscreen interfaceUniversal electroporation for transfection of cells in vitro, in vivo, in ovo, and adherent formsPreset protocols include the most common eukaryotic and prokaryotic cell typesUser-defined protocols: protocols can be added and modified without limitsSafety functions: Pre-pulse resistance check, arc protection, and over-current pulse abortData protocols store parameters of all delivered pulses for quality control and troubleshooting purposesStorage of over 1000 protocolsScope of supply: Generator Gemini SCExponential decay pulseCapacitance LV mode:25 ... 3275 µFCapacitance HV mode:10, 25, 50 µFResistance:50 ... 1000 ΩVoltage LV mode:10 ... 500 VVoltage HV mode:510 ... 3000 VSquare wave pulseCapacitance LV mode:3775 µFCapacitance HV mode:85 µFVoltage LV mode:10 ... 500 VVoltage HV mode:510 ... 3000 VMultiple pulse LV mode:1 ... 10 pulses per sampleMultiple pulse HV mode:1 ... 2 pulses per samplePulse length LV mode:0.05 ... 100 msPulse length HV mode:50 µs ... 5 msPulse interval:0.1 ... 10 sCharging time LV mode:7 sCharging time HV mode:4 s
Electroporation system Gemini Twin Wave X2, Complete system
The Gemini Twin Wave devices are flexible systems that can generate both square wave and exponential decay waves. Ideally suited for all electroporation requirements. The waveform combination allows eukaryotic and prokaryotic cells to be electroporated easily and efficiently in cuvettes or plates with a single device. Prokaryotic cells typically respond well to exponential decay wave pulses, while eukaryotic cells are most efficiently transfected with square wave pulses.Large, user-friendly touchscreen interfaceUniversal electroporation for transfection of cells in vitro, in vivo, in ovo, and adherent formsPreset protocols include the most common eukaryotic and prokaryotic cell typesUser-defined protocols: protocols can be added and modified without limitsSafety functions: Pre-pulse resistance check, arc protection, and over-current pulse abortData protocols store parameters of all delivered pulses for quality control and troubleshooting purposesStorage of over 1000 protocolsThe supplied safety dome connects to the generator to safely deliver high-voltage electrical pulses to cuvettes. Up to two cuvettes can be electroporated simultaneously.Scope of supply: Generator Gemini X2, safety dome, electroporation cuvettes 1 mm / 2 mm / 4 mm (10 each), cuvette rackExponential decay pulseCapacitance LV mode:25 ... 3275 µFCapacitance HV mode:10, 25, 35, 50, 60, 75, 85 µFResistance LV mode:25 ... 1575 ΩResistance HV mode:50 ... 1575 ΩVoltage LV mode:5 ... 500 VVoltage HV mode:505 ... 3000 VMultiple pulse:1 ... 2 (depending on amplitude limit of the device)Pulse interval:5 ... 30 sSquare wave pulseCapacitance LV mode:3775 µFCapacitance HV mode:85 µFVoltage LV mode:5 ... 500 VVoltage HV mode:505 ... 3000 VMultiple pulse single sample:1 ... 99 pulses per sampleMultiple pulse plate:1 ... 10 pulses per samplePulse length LV mode:10 µs ... 999 msPulse length HV mode:10 ... 600 µsPulse interval:0.1 ... 10 sCharging time LV mode:7 sCharging time HV mode:4 s
Electroporation system Gemini Twin Wave X2, Generator
The Gemini Twin Wave devices are flexible systems that can generate both square wave and exponential decay waves. Ideally suited for all electroporation requirements. The waveform combination allows eukaryotic and prokaryotic cells to be electroporated easily and efficiently in cuvettes or plates with a single device. Prokaryotic cells typically respond well to exponential decay wave pulses, while eukaryotic cells are most efficiently transfected with square wave pulses.Large, user-friendly touchscreen interfaceUniversal electroporation for transfection of cells in vitro, in vivo, in ovo, and adherent formsPreset protocols include the most common eukaryotic and prokaryotic cell typesUser-defined protocols: protocols can be added and modified without limitsSafety functions: Pre-pulse resistance check, arc protection, and over-current pulse abortData protocols store parameters of all delivered pulses for quality control and troubleshooting purposesStorage of over 1000 protocolsScope of supply: Generator Gemini X2Exponential decay pulseCapacitance LV mode:25 ... 3275 µFCapacitance HV mode:10, 25, 35, 50, 60, 75, 85 µFResistance LV mode:25 ... 1575 ΩResistance HV mode:50 ... 1575 ΩVoltage LV mode:5 ... 500 VVoltage HV mode:505 ... 3000 VMultiple pulse:1 ... 2 (depending on amplitude limit of the device)Pulse interval:5 ... 30 sSquare wave pulseCapacitance LV mode:3775 µFCapacitance HV mode:85 µFVoltage LV mode:5 ... 500 VVoltage HV mode:505 ... 3000 VMultiple pulse single sample:1 ... 99 pulses per sampleMultiple pulse plate:1 ... 10 pulses per samplePulse length LV mode:10 µs ... 999 msPulse length HV mode:10 ... 600 µsPulse interval:0.1 ... 10 sCharging time LV mode:7 sCharging time HV mode:4 s
Electroporation system Gemini Twin Wave X2, HT system
The Gemini Twin Wave devices are flexible systems that can generate both square wave and exponential decay waves. Ideally suited for all electroporation requirements. The waveform combination allows eukaryotic and prokaryotic cells to be electroporated easily and efficiently in cuvettes or plates with a single device. Prokaryotic cells typically respond well to exponential decay wave pulses, while eukaryotic cells are most efficiently transfected with square wave pulses.Large, user-friendly touchscreen interfaceUniversal electroporation for transfection of cells in vitro, in vivo, in ovo, and adherent formsPreset protocols include the most common eukaryotic and prokaryotic cell typesUser-defined protocols: protocols can be added and modified without limitsSafety functions: Pre-pulse resistance check, arc protection, and over-current pulse abortData protocols store parameters of all delivered pulses for quality control and troubleshooting purposesStorage of over 1000 protocolsWith the supplied HT-200 plate handler, 96-well electroporation plates can be processed. The plate handler delivers a single pulse to the wells of the plate, using the parameters set in the generator.Scope of supply: Generator Gemini X2, safety dome, electroporation cuvettes 1 mm / 2 mm / 4 mm (10 each), cuvette rack, HT-200 plate handler, electroporation plate 2 mm and 4 mm gapExponential decay pulseCapacitance LV mode:25 ... 3275 µFCapacitance HV mode:10, 25, 35, 50, 60, 75, 85 µFResistance LV mode:25 ... 1575 ΩResistance HV mode:50 ... 1575 ΩVoltage LV mode:5 ... 500 VVoltage HV mode:505 ... 3000 VMultiple pulse:1 ... 2 (depending on amplitude limit of the device)Pulse interval:5 ... 30 sSquare wave pulseCapacitance LV mode:3775 µFCapacitance HV mode:85 µFVoltage LV mode:5 ... 500 VVoltage HV mode:505 ... 3000 VMultiple pulse single sample:1 ... 99 pulses per sampleMultiple pulse plate:1 ... 10 pulses per samplePulse length LV mode:10 µs ... 999 msPulse length HV mode:10 ... 600 µsPulse interval:0.1 ... 10 sCharging time LV mode:7 sCharging time HV mode:4 s
Enduro™ power supplies
Dependable performance, intelligent control, for the connection of different chamber systems. Ideal for DNA, RNA and protein electrophoresis as well as blotting.Simple to programSelect constant voltage or constant current modeCompact, lightweight and stackableExtremely quiet operationSpecificationsModel 300 VOutput voltage range/increments:2 ... 300 V/1 V 4 ... 500 mA/1 mA 90 W max/naTimer:1 min to 99 h 59 min or continuousProgrammable:NoDimensions:190 x 250 x 80 mmWeight:2.2 kgInput voltage:120-230 VOutput voltage range/increments:5 ... 250 V/1 V 10 ... 3,000 mA/10 mA 1 ... 300 W/1 WTimer:1 min to 99 h 59 min or continuousProgrammable:10 steps/program, 20 program memoryDimensions:190 x 250 x 80 mmWeight:2.5 kgInput voltage:120 ... 230 VAdditional accessories are available.
Ethidium Bromide Extractor
For the rapid and safe removal of ethidium bromide from gel-staining solutions. Contains an activated carbon matrix, which quickly and easily removes >99 % of ethidium bromide. Each device can decontaminate up to 10 litres of gel-staining solution. After filtration, the decontaminated solution can be safely poured down the laboratory drain. The extractor funnel device fits most standard laboratory flasks and bottles (33 to 45 mm necks), and the unit includes a cap for storage between uses. Pack of 2
Fast PCR-Tubes 0.1 ml, 8 tube-strips with separate cap strip
The Fast PCR tube strips made of polyethylene transfer heat much better and faster than tubes made of traditional plastics. They are particularly suitable for use with Fast PCR reagent kits and for cyclers with high heating and cooling rates. Under these conditions, the following results higher yield and significant time savings.Suitable for PCR, Fast PCR and Real Time PCRIncreased thermal conductivity through PE tubesHigher yield and significant time savingsCan be used in any cycler for 100 µL volumesLid strip PPWith flat or domed lidCertified PCR clean
FTA Card Accessories
FTA Purification ReagentRemoves haem, PCR inhibitors, and other potential contaminants to ensure superior quality DNA for downstream analysis.Sterile Foam Tipped ApplicatorEasy-to-use applicator for the noninvasive collection and transfer of buccal cells to FTA Cards.Multi-Barrier PouchesFor transporting or storing FTA Cards. Protects cards from environmental contamination. Tamper-evident seals maintain sample security for forensic samples. A resealable pouch is also available when multiple access to FTA cards is needed.Storage Desiccant PacketsEnsure that FTA Cards remain dry during transport or storage. Contains indicator that changes colour to verify moisture adsorption.
FTA Cards
FTA Classic CardsFour sample areas for storage of up to 4 x 125µl whole blood or 4 x 75µl plant homogenate per card. Convenient for multiple applications of the same specimen or collection of multiple animal or plant samples. Also available in Indicating (pink) FTA format.FTA Mini CardTwo sample areas for storage of up to 2 x 125µl whole blood or 2 x 75µl plant homogenate per card. Convenient for protocols that require different locations for testing and archiving samples. Also available in Indicating (pink) FTA format.FTA Micro CardOne sample area for storage of up to 125µl whole blood or 75µl plant homogenate per card. Recommended when only one sample is needed. Also available in Indicating (pink) FTA format.FTA Gene CardThree sample areas in a card frame for storage of up to 3 x 75µl whole blood or 3 x 50µl plant homogenate per card. Can be run in most automatic dispensing/pipetting systems when used with the FTA Gene Card Tray.Indicating FTA Cards:With colour indicator that changes from pink to white when sample is applied. useful for colourless samples.
Gel / PCR / DNA Small Fragment Extraction Kit
The test kit was developed to obtain or concentrate DNA fragments from agarose gels, PCR or other enzymatic processes.The agarose gel is dissolved, the enzymes are denatured and the DNA fragments will bind to the glass fibre matrix in the spin column. Wash buffers (containing ethanol) are used to remove contaminants and a low salt elution buffer is used to recover the purified DNA fragments. Recoveries are up to 90 % for gel extraction and up to 95 % for PCR clean-up. With this kit, PCR purification and gel extraction procedures can be performed, so that a second test kit is not necessary.High recovery rateShort process timeOne test kit for two methodsSpecificationsSample size:up to 300 mg of agarose gel / up to 100 µl of PCR productElution volume:20-50 µlFragment size:50 bp - 200 bpTypical yield:90-95 % gel extraction and PCR clean-upOperation Time:20 min.
Gel Documentation System Doc-Print CX3
Doc-Print CX3 is a stand-alone system with a small footprint that doesn't need a computer. The free VisionCapt software guarantees full GLP compliance. Molecular weight calculation, band quantification, colony counting, distance calculation, text annotations and image enhancement are included.Field of view: 24 x 18 cmScientific grade camera - Made in Germany3 megapixel resolution (extandable to 20 megapixels)Auto-exposure16-bit - 65 536 grey levelsPassive coolingUSB 3 connectionLarge 12.6" touchscreen interface displaySelf-understandable user interfacePrint or save on an external drive via the USB portConnection of a thermal printer possibleAll models include the following standard components: Imaging unit with touch display and software, camera, zoom objective, UV-/vis F-590 Filter and dark hood.