
Accessories and replacement parts for EC columns
Analytical column system manufactured from stainless steel M 8 outer threads on both ends combination of sealing element and very fine-meshed stainless steel screen, PTFE sealing ring and fitting adaptor column heads SW 12 with inner threads M8 x 0,75 and UNF 10-32, as built -in guard columns use ChromCart® guard column cartridges with 8mm length with the guard column adaptor EC, packed with NUCLEODUR® spherical silica.
EC analytical columns NUCLEOCEL DELTA S, 5 µm
NUCLEOCEL DELTA enantiomer separation based on cellulose derivativesBase material silica,chiral selector Cellulosetris-(3,5-dimethylphenylcarbamate) - USP L40Similar phases: Chiralcel® OD, Kromasil®, CelluCoat™, Eurocel® 01S-Type for high resolution, allows use of shorter columns (150 mm) for faster separations, pressure stability up to ~ 150 bar (2000 psi).Recommended applications: pharmaceutically active compounds, chiral pollutants (e. g. herbicides, PCB), chiral compounds in food (dyes, preservatives), chiral catalysts and bioorganic compounds.NUCLEOCEL DELTA for normal phase applications:Eluent in column n-heptane - propanol-2 (90:10, v/v), particle size 5 µm.
EC analytical columns NUCLEOCEL DELTA-RP S, 5 µm
NUCLEOCEL DELTA enantiomer separation based on cellulose derivativesBase material silica,chiral selector Cellulosetris-(3,5-dimethylphenylcarbamate) - USP L40Similar phases: Chiralcel® OD, Kromasil®, CelluCoat™, Eurocel® 01S-Type for high resolution, allows use of shorter columns (150 mm) for faster separations, pressure stability up to ~ 150 bar (2000 psi).Recommended applications: pharmaceutically active compounds, chiral pollutants (e. g. herbicides, PCB), chiral compounds in food (dyes, preservatives), chiral catalysts and bioorganic compounds.NUCLEOCEL DELTA-RP for reversed phase applications:Eluent in column acetonitrile - water (40:60, v/v), particle size 5 µm. Designed for use either in polar organic mode or with eluents containing high concentrations of chaotropic salts such as perchlorate.
EC analytical columns NUCLEODUR® 100-3
Totally spherical high purity silica - USP L3 pore size 110 Å, pore volume 0.9 ml/g, surface (BET) 340 m²/g, density 0.47 g/ml, pressure stability 800 bar, pH stability 2 - 8.Particle size 3 µm.

EC analytical columns NUCLEODUR® 100-3 C18 ec, 3 µm
Available with medium density octadecyl (C18 - USP L1) and octyl (C8 - USP L7) modification pore size 110 Å, particle sizes 3 µm and 5 µm; 7 µm, 10 µm, 12 µm, 16 µm, 20 µm, 30 µm und 50 µm for preparative separations on request for daily routine analysis and up-scaling for preparative HPLC, pH stability 1 - 9. Carbon content 17.5 % C for C18, 10.5 % C for C8, high reproducibility from lot to lot for standard routine applications in reversed phase chromatography.Octadecyl phases, 17.5 % C, particle size 3 µm.
EC analytical columns NUCLEODUR® 100-3 C8 ec, 3 µm
Available with medium density octadecyl (C18 - USP L1) and octyl (C8 - USP L7) modification pore size 110 Å, particle sizes 3 µm and 5 µm; 7 µm, 10 µm, 12 µm, 16 µm, 20 µm, 30 µm und 50 µm for preparative separations on request for daily routine analysis and up-scaling for preparative HPLC, pH stability 1 - 9. Carbon content 17.5 % C for C18, 10.5 % C for C8, high reproducibility from lot to lot for standard routine applications in reversed phase chromatography.Octyl phases, 10.5 % C, particle size 3 µm.
EC analytical columns NUCLEODUR® 100-3 CN-RP, 3 µm
Pore size 110 Å, - USP L10Multi-mode columns (RP and NP)Widens the scope in selectivityDiffernet retention characteristics compared to C8 and C18Stable against hydrolysis at low pH values, working range pH 1-8High reproducibility from lot to lotIdeal for: tricyclic antidepressants, steroids, organic acidsEluent in column acetonitrile, particle size 3 µm.
EC analytical columns NUCLEODUR® 100-3 NH2-RP, 3 µm
Pore size 110 Å, particle sizes 3 and 5 µm; 2.5 % C; not endcapped - USP L8Multi-mode columns (RP and NP):Normal phase chromatography (NP) with hexan, dichloromethane or 2-propanol as mobile phase for polar compounds such as substituted anilines, esters, chlorinated pesticidesReversed phase chromatography (RP) of polar compounds like sugars in aqueous-organic eluent systemsIon exchange chromatography of anions and organic acids using common buffers and organic modifiersstable against hydrolysis at low pH, working range pH 2-8, 100 % stable in water, suitable for LC-MSIdeal for:polar compounds under RP conditions (sugars, DNA bases), hydrocarbons under NP conditionsEluent in column is n-heptane for the NP mode - RP columns are delivered in acetonitrile - water. For changing the solvent system a rinsing step with THF may be necessary.Eluent in column acetonitrile, particle size 3 µm.
EC analytical columns NUCLEODUR® 100-5
Totally spherical high purity silica - USP L3 pore size 110 Å, pore volume 0.9 ml/g, surface (BET) 340 m²/g, density 0.47 g/ml, pressure stability 800 bar, pH stability 2 - 8.Particle size 5 µm.

EC analytical columns NUCLEODUR® 100-5 C18 ec, 5 µm
Available with medium density octadecyl (C18 - USP L1) and octyl (C8 - USP L7) modification pore size 110 Å, particle sizes 3 µm and 5 µm; 7 µm, 10 µm, 12 µm, 16 µm, 20 µm, 30 µm und 50 µm for preparative separations on request for daily routine analysis and up-scaling for preparative HPLC, pH stability 1 - 9. Carbon content 17.5 % C for C18, 10.5 % C for C8, high reproducibility from lot to lot for standard routine applications in reversed phase chromatography.Octadecyl phases, 17.5 % C, particle size 5 µm.

EC analytical columns NUCLEODUR® 100-5 C8 ec, 5 µm
Available with medium density octadecyl (C18 - USP L1) and octyl (C8 - USP L7) modification pore size 110 Å, particle sizes 3 µm and 5 µm; 7 µm, 10 µm, 12 µm, 16 µm, 20 µm, 30 µm und 50 µm for preparative separations on request for daily routine analysis and up-scaling for preparative HPLC, pH stability 1 - 9. Carbon content 17.5 % C for C18, 10.5 % C for C8, high reproducibility from lot to lot for standard routine applications in reversed phase chromatography.Octyl phases, 10.5 % C, particle size 5 µm.
EC analytical columns NUCLEODUR® 100-5 CN, 5 µm
Pore size 110 Å, - USP L10Multi-mode columns (RP and NP)Widens the scope in selectivityDiffernet retention characteristics compared to C8 and C18Stable against hydrolysis at low pH values, working range pH 1-8High reproducibility from lot to lotIdeal for: tricyclic antidepressants, steroids, organic acidsEluent in column n-heptane, particle size 5 µm.
EC analytical columns NUCLEODUR® 100-5 CN-RP, 5 µm
Pore size 110 Å, - USP L10Multi-mode columns (RP and NP)Widens the scope in selectivityDiffernet retention characteristics compared to C8 and C18Stable against hydrolysis at low pH values, working range pH 1-8High reproducibility from lot to lotIdeal for: tricyclic antidepressants, steroids, organic acidsEluent in column acetonitrile, particle size 5 µm
EC analytical columns NUCLEODUR® 100-5 NH2, 5 µm
Pore size 110 Å, particle sizes 3 and 5 µm; 2.5 % C; not endcapped - USP L8Multi-mode columns (RP and NP):Normal phase chromatography (NP) with hexan, dichloromethane or 2-propanol as mobile phase for polar compounds such as substituted anilines, esters, chlorinated pesticidesReversed phase chromatography (RP) of polar compounds like sugars in aqueous-organic eluent systemsIon exchange chromatography of anions and organic acids using common buffers and organic modifiersstable against hydrolysis at low pH, working range pH 2-8, 100 % stable in water, suitable for LC-MSIdeal for:polar compounds under RP conditions (sugars, DNA bases), hydrocarbons under NP conditionsEluent in column is n-heptane for the NP mode - RP columns are delivered in acetonitrile - water. For changing the solvent system a rinsing step with THF may be necessary.Eluent in column n-heptane, particle size 5 µm.
EC analytical columns NUCLEODUR® 100-5 NH2-RP, 5 µm
Pore size 110 Å, particle sizes 3 and 5 µm; 2.5 % C; not endcapped - USP L8Multi-mode columns (RP and NP):Normal phase chromatography (NP) with hexan, dichloromethane or 2-propanol as mobile phase for polar compounds such as substituted anilines, esters, chlorinated pesticidesReversed phase chromatography (RP) of polar compounds like sugars in aqueous-organic eluent systemsIon exchange chromatography of anions and organic acids using common buffers and organic modifiersstable against hydrolysis at low pH, working range pH 2-8, 100 % stable in water, suitable for LC-MSIdeal for:polar compounds under RP conditions (sugars, DNA bases), hydrocarbons under NP conditionsEluent in column is n-heptane for the NP mode - RP columns are delivered in acetonitrile - water. For changing the solvent system a rinsing step with THF may be necessary.Eluent in column acetonitrile, particle size 5 µm.

EC analytical columns NUCLEODUR® C18 Gravity, 1.8 µm
NUCLEODUR® C18 - C 8 Gravity nonpolar high density phasesAvailable as octadecyl (C18 -USP L1) and octyl (C8 - USP L7) modifications. Pore size 110 Å.Ideal for method developmentAllows HPLC at pH extremes (pH 1 - 11)Suitable for LC/MS due to low bleeding characteristicsRecommended for overall sophisticated analytical separationsOptimal for: pharmaceuticals, e.g. analgesics, antiinflammatory drugs, antidepressants, herbicides, phytopharmaceuticals, immunosppressantsParticle size 1.8 µm, 18 % C

EC analytical columns NUCLEODUR® C18 Gravity, 3 µm
NUCLEODUR® C18 - C 8 Gravity nonpolar high density phasesAvailable as octadecyl (C18 -USP L1) and octyl (C8 - USP L7) modifications. Pore size 110 Å.Ideal for method developmentAllows HPLC at pH extremes (pH 1 - 11)Suitable for LC/MS due to low bleeding characteristicsRecommended for overall sophisticated analytical separationsOptimal for: pharmaceuticals, e.g. analgesics, antiinflammatory drugs, antidepressants, herbicides, phytopharmaceuticals, immunosppressantsParticle size 3 µm, 18 % C.

EC analytical columns NUCLEODUR® C18 Gravity, 5 µm
NUCLEODUR® C18 - C 8 Gravity nonpolar high density phasesAvailable as octadecyl (C18 -USP L1) and octyl (C8 - USP L7) modifications. Pore size 110 Å.Ideal for method developmentAllows HPLC at pH extremes (pH 1 - 11)Suitable for LC/MS due to low bleeding characteristicsRecommended for overall sophisticated analytical separationsOptimal for: pharmaceuticals, e.g. analgesics, antiinflammatory drugs, antidepressants, herbicides, phytopharmaceuticals, immunosppressantsParticle size 5 µm, 18 % C
EC analytical columns NUCLEODUR® C18 Isis, 1.8 µm
C18 phase with special polymeric, crosslinked surface modification - USP L1Pore size 110 ÅHigh steric selectivityOutstanding surface deactivationSuitable for LC/MS due low bleeding characteristicspH stability 1 - 10Broad range of application: steroids, (o,p,m-) substituted aromatics, fat-soluble vitaminsParticle size 1.8 µm, 20 % C
EC analytical columns NUCLEODUR® C18 Isis, 3 µm
C18 phase with special polymeric, crosslinked surface modification - USP L1Pore size 110 ÅHigh steric selectivityOutstanding surface deactivationSuitable for LC/MS due low bleeding characteristicspH stability 1 - 10Broad range of application: steroids, (o,p,m-) substituted aromatics, fat-soluble vitaminsParticle size 3 µm, 20 % C
EC analytical columns NUCLEODUR® C18 Isis, 5 µm
C18 phase with special polymeric, crosslinked surface modification - USP L1Pore size 110 ÅHigh steric selectivityOutstanding surface deactivationSuitable for LC/MS due low bleeding characteristicspH stability 1 - 10Broad range of application: steroids, (o,p,m-) substituted aromatics, fat-soluble vitaminsParticle size 5 µm, 20 % C
EC analytical columns NUCLEODUR® C18 Pyramid, 1.8 µm
Stable in 100 % aqueous eluent systems- USP L1Pore size 110 ÅInteresting polar selectivity featuresExcellent base deactivation; suitable for LC/MS due low bleeding characteristicspH stability 1 - 9Ideal for: analgesics, penicillin antibiotics, nucleic acid bases, water-soluble vitamins, complexing agetns, organic acidsParticle size 1.8 µm, 14 % C.
EC analytical columns NUCLEODUR® C18 Pyramid, 3 µm
Stable in 100 % aqueous eluent systems- USP L1Pore size 110 ÅInteresting polar selectivity featuresExcellent base deactivation; suitable for LC/MS due low bleeding characteristicspH stability 1 - 9Ideal for: analgesics, penicillin antibiotics, nucleic acid bases, water-soluble vitamins, complexing agetns, organic acidsParticle size 3 µm, 14 % C
EC analytical columns NUCLEODUR® C18 Pyramid, 5 µm
Stable in 100 % aqueous eluent systems- USP L1Pore size 110 ÅInteresting polar selectivity featuresExcellent base deactivation; suitable for LC/MS due low bleeding characteristicspH stability 1 - 9Ideal for: analgesics, penicillin antibiotics, nucleic acid bases, water-soluble vitamins, complexing agetns, organic acidsParticle size 5 µm, 14 % C